• Mi-T-MEF™ Mouse Feeder Cells
• MouseEZ™ Target Cells

Maintaining embryonic stem cells or induced pluripotent stem cells in a pluripotent, self-renewing and karyotypically normal state for a reasonable and defined period of time currently requires culturing ESC or iPSC in contact with established feeder cells such as mouse embryonic fibroblasts (MEFs).  ArunA Biomedical's Mi-T-MEF™ Mouse Feeder Cells are high quality cells that are mitotically inactivated through mitomycin C treatment and ready to use.  They have been prequalified for human embryonic stem cell work and provide an easy to use source of feeder layers to meet your stem cell needs.

Cell Type:   primary mouse embryonic fibroblast cells
Part Number:  A-6
Species:  Mus musculus
Source:  ICR strain
Morphology:  fibroblast
Quantity:  >5x10⁶ cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties: adherent
Medium:  AB MEF Medium

Pathogen testing:    Product #A-6 has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV and shown to be negative (performed by GIVF Laboratories). Cells are also tested for sterility and mycoplasma contamination.

Viability testing:  Post-cryostorage viability was determined to be approximately 75%. 

Kit format:

Mitomycin-C Inactivated Mouse Feeder Cells for Microenvironment Support to Pluripotent Stem Cells

• Mi-T-MEF™ Mouse Feeder Cells (Cat#A-6), 3 vials (>5 x 10⁶ cells)

Technical information:

Product Insert for Mi-T-MEF™ Mouse Feeder Cells (PDF)

Mi-T-MEF™ Mouse Feeder Cells include studies of:

• Microenvironment Support for Generation and Maintenance of Induced Pluripotent Stem Cells
   
• Microenvironment Support for Generation and Maintenance of Human Germ Cells 

• Microenvironment Support for Maintenance of Pluripotent Human and Mouse Embryonic Stem Cells

Mouse Feeder Cell references and publications can be found here.

Products are to be used for research purposes only, not for use in diagnostic or therapeutic applications. Products may not be sold, modified for sale or used for commercial purposes.

If you are having difficulties with using our products, please check our Frequently Asked Questions and/or contact us. 

I only obtained 50% recovery from the frozen feeder cells (Cat #A-6), what should I do?

Feeder density used for culturing pluripotent stem cells varies from lab to lab.  Feeders at a
density as low as 1 x 10⁶ cells per 10 cm dish have been successfully used to culture human
embryonic stem (hES) cells (this is the density used by the Harvard Stem Cell Institute). Thus, you could still potentially use the feeder cells even if you got 50% recovery.  

Products are to be used for research purposes only, not for use in diagnostic or therapeutic applications. Products may not be sold, modified for sale or used for commercial purposes. 

 Left, Phase contrast image of mitomycin C inactivated AB Mi-T-MEF™ Mouse Feeder Cells (Cat# A-6) in culture. Right, Epiflourescent image of transcriptionally active AB MouseEZ™ (Cat#A-7) following transduction with viPS™ - EF1α-TurboGFP lentiviral vector.